Russian "successful" clone B0/W148 of Mycobacterium tuberculosis Beijing genotype: a multiplex PCR assay for rapid detection and global screening.

نویسندگان

  • Igor Mokrousov
  • Olga Narvskaya
  • Anna Vyazovaya
  • Tatiana Otten
  • Wei-Wei Jiao
  • Lia Lima Gomes
  • Philip N Suffys
  • A-Dong Shen
  • Boris Vishnevsky
چکیده

We describe a multiplex PCR assay to detect the Mycobacterium tuberculosis Beijing genotype variant B0/W148, which is considered a "successful" clone of M. tuberculosis, widespread in Russia. Specificity and sensitivity of the assay were 100% based on the analysis of a collection of 516 M. tuberculosis isolates of different genotypes and origins. This assay may be used for accurate and simple detection and surveillance of this clinically and epidemiologically important variant of M. tuberculosis.

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Russian ‘ successful ’ clone В 0 / W 148 of Mycobacterium tuberculosis Beijing genotype : 1 multiplex PCR assay for rapid detection and global screening

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 50 11  شماره 

صفحات  -

تاریخ انتشار 2012